製品の概要

  • 製品名DHEA sulfate (DHEA-S) ELISA Kit
    DHEA-S キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Control 64 < 5.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Control 48 < 9.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    = 0.03 ng/ml
  • 検出範囲
    0.1 µg/ml - 10 µg/ml
  • 添加回収試験

    = 96.71 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.

     

    A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    5X Serum Diluent 1 x 20ml
    Anti-DHEA sulfate IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover foils 1 unit
    DHEA sulfate control 1 x 1ml
    DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml
    DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml
    DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml
    DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml
    DHEA sulfate-HRP Conjugate 1 x 12ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab108669 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Competitive ELISA Use at an assay dependent dilution.

    DHEA sulfate (DHEA-S) ELISA Kit 画像

    • Representative Standard Curve using ab108669

    プロトコール

    DHEA sulfate (DHEA-S) ELISA Kit (ab108669) 使用論文

    ab108669 has not yet been referenced specifically in any publications.

    Product Wall

    The standard range of 0.1 - 10 ug/ml takes into account the 1/50 sample dilution that is done, so you do not need to multiply your end measurement by 50.

    DHEA-S and DHEA are very similar molecules: our antibody has more affinity for DHEA compared to DHEA-S (10 % higher). You have to consider that in human blood the quantity of DHEA is 1.000 times lower than DHEA-S (from clinical point of view the elevat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"