製品の概要

  • 製品名
    Anti-DHEA 3-Sulfate antibody
    DHEA 3-Sulfate 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to DHEA 3-Sulfate
  • 特異性
    Cross reactivity : DHEAS 100% DHEA 69% 5-alpha-Androstendione 5.3% 5 Androstendione 3.6% 4 Androstendione 2.3% Androstendione 3.0% Testosterone <0.3% Androstendiol <0.4%
  • アプリケーション
    適用あり: RIAmore details
  • 免疫原

    DHEA 3-Sulfate conjugated to HMS-BSA

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Blue Sepharose chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
RIA
  • 追加情報
    RIA: 100 µl/test (3H).

    Assay buffer : 0.01 M PBS, pH 7.4 with 0.1% gelatin and 0.1% thiomersal.

    Add 500 µl buffer and divide into 100 µl aliquots for storage at -20ºC. When required, dilute each aliquot to 10 ml using assay buffer. Use 100 µl/test.

    Use Dextran coated charcoal to separate.

    The antiserum is sufficiently specific and does not require pre-assay chromatographic purification. No extraction of the sample is required.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      DHEA (Dehydroepiandrosterone) is an endogenous steroid hormone made in the human body, and secreted by the adrenal gland. DHEA serves as precursor to male and female sex hormones (androgens and estrogens). Dehydroepiandrosterone sulfate (DHEAS) is the sulfated version of DHEA. This conversion is reversibly catalysed by sulfotransferase (SULT2A1) primarily in the adrenals, the liver, and small intestines. In blood, most DHEA is found as DHEAS with levels that are about 300 times higher than free DHEA. While DHEA levels reach their peak in the early morning hours, DHEAS levels show no diurnal variation. DHEAS is biologically active only after its sulfate group has been split and it becomes DHEA again. Measurement of DHEAS is preferable to DHEA as levels are more stable.
    • 細胞内局在
      Secreted
    • 別名
      • Dehydroepiandrosterone sulfate antibody

    参考文献

    ab36365 has not yet been referenced specifically in any publications.

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