製品の概要

  • 製品名Anti-DHCR7 antibody
  • 製品の詳細
    Rabbit polyclonal to DHCR7
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fragment protein corresponding to amino acids 1-259 of Human DHCR7 (NP_001351.2).

  • ポジティブ・コントロール
    • Rat brain tissue lysate, Mouse testis tissue lysate, HepG2 cell lysate IF/ICC: HeLa cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.

ターゲット情報

  • 関連性The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
  • 細胞内局在Endoplasmic reticulum membrane; Multi-pass membrane protein
  • 参照データベース
  • 別名
    • 7 dehydrocholesterol reductase antibody
    • 7 DHC reductase antibody
    • Delta 7 dehydrocholesterol reductase antibody
    • Putative sterol reductase SR 2 antibody
    • SLOS antibody
    • Sterol delta 7 reductase antibody
    • Sterol Delta antibody
    see all

Anti-DHCR7 antibody 画像

  • ICC/IF image of ab103296 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103296, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DHCR7 antibody (ab103296) at 1/500 dilution + Rat brain tissue lysate at 50 µg

    Predicted band size : 54 kDa
  • Anti-DHCR7 antibody (ab103296) at 1/500 dilution + Mouse testis tissue lysate at 50 µg

    Predicted band size : 54 kDa
  • Anti-DHCR7 antibody (ab103296) at 1/500 dilution + HepG2 cell lysate at 50 µg

    Predicted band size : 54 kDa

Anti-DHCR7 antibody (ab103296) 使用論文

This product has been referenced in:
  • Fryer LG  et al. The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis. J Biol Chem 289:4244-61 (2014). WB ; Human . Read more (PubMed: 24338480) »
  • Sugiyama K  et al. Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus. PLoS One 9:e94460 (2014). WB, IF ; Human . Read more (PubMed: 24718268) »

See all 3 Publications for this product

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Thank you for your reply.

Your credit note ID is*/.

I am sorry that this antibody did not perform as stated on the datasheet and that we were not able to get it working for you. If payment has already been made on the original order a...

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Thank you for your reply.

I am sorry that you are still experiencing problems with ab103296 in western blotting.

To answer your questions:

1 - There is already an image showing the antibody react with HepG2 cells (human liver...

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You should have the new vial of ab103296 to try out tomorrow morning. Fingers crossed that it works for you. If not, please let me know (or if does work, I would like to know aswell).

Hope you are having a great day.

Thank you for your reply and for sending those western blot images.

I am very sorry that you are having issues with this antibody and I cannot think of anything else that would help resolve this issue. It could be that something happened to th...

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Thank you for your reply.

I am going to send the tissue lysate for you to use as a positive control in your western blots. The lysate is currently out of stock, but we are due to get it in on Friday (15th), so that means we should hopefully g...

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Thank you for your reply.

Based on the information that you have provided, I think that the method of tissue preparation is fine, I don’t think that could be the issue. One piece of protocol that is different between what you do and the...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab103296. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on Mouse, Rat and Human samples. If we cannot...

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Thanky you for your enquiry. I'm sorry to hear the vial of ab103296 you received is giving you trouble. The western blots shown on the online datasheet for ab103296 illustrate detection of DHCR7 from Rat brain tissue lysate, mouse testis tissue lysate ...

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Thank you for your enquiry. I can confirm the immunogen for ab103296 Anti-DHCR7 antibody is DHCR7 full-length human protein (NP_001351.1, amino acids 1 ~ 259), and its expression system is mammalian cell. I can confirm this item is sourced e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"