The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 124 kDa).
Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
Belongs to the eukaryotic diacylglycerol kinase family. Contains 2 ANK repeats. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.