The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
ab71735, at a dilution of 1/50, staining DGKI in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71735 was peroxidase conjugated to the secondary antibody, followed by AEC staining.