製品の概要

  • 製品名Anti-DGKH antibody
    DGKH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DGKH
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human DGKH

  • ポジティブ・コントロール
    • Fetal heart lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).Can be blocked with Human DGKH peptide (ab99354). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/500.

ターゲット情報

  • 関連性This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family of proteins, specifically the type II DGK subfamily. Members of this family are involved in regulating the intracellular concentrations of diacylglycerol and phosphatidic acid. Two transcript variants encoding distinct isoforms have been identified for this gene.
  • 細胞内局在Cytoplasm. Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association.
  • 参照データベース
  • 別名
    • DAG kinase eta antibody
    • DGK eta antibody
    • Diacylglycerol kinase eta antibody
    • Diglyceride kinase eta antibody

Anti-DGKH antibody 画像

  • Sample Type : Adult mouse cortex. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti-rabbit-Cy3. Secondary Antibody Dilution : 1:1000. Color/Signal Descriptions : Red: DGKH Cyan: Nissl (Neurons). Gene Name : DGKH.
  • Anti-DGKH antibody (ab80693) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Gel concentration 6-18%

Anti-DGKH antibody (ab80693) 使用論文

This product has been referenced in:
  • Martinez K  et al. Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. J Lipid Res 54:662-70 (2013). Human . Read more (PubMed: 23264678) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab80693.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"