製品の概要

  • 製品名Anti-DGKA antibody
    DGKA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DGKA
  • 特異性This antibody detects endogenous levels of total DGKA protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 331-370 of Human DGKA

  • ポジティブ・コントロール
    • Extracts from Jurkat cells. IHC-P (FFPE): Human Skin (Normal) tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab64845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
  • 組織特異性Lymphocytes and oligodendroglial cells.
  • 配列類似性Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 EF-hand domains.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 80 kDa diacylglycerol kinase antibody
    • DAG kinase alpha antibody
    • DAGK antibody
    • DAGK1 antibody
    • DGK alpha antibody
    • DGK-alpha antibody
    • dgkA antibody
    • DGKA_HUMAN antibody
    • Diacylglycerol kinase alpha antibody
    • Diacylglycerol kinase, alpha 80kDa antibody
    • Diglyceride kinase alpha antibody
    • MGC12821 antibody
    • MGC42356 antibody
    • OTTHUMP00000242836 antibody
    • OTTHUMP00000242955 antibody
    • OTTHUMP00000244046 antibody
    see all

Anti-DGKA antibody 画像

  • IHC image of DGKA staining in Human Skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64845, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-DGKA antibody (ab64845) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ICC/IF image of ab64845 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64845, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DGKA antibody (ab64845) 使用論文

ab64845 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"