製品の概要

  • 製品名Anti-DGCR8 antibody
    DGCR8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DGCR8
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of Human DGCR8 (NP_073557.3).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab90579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 WW domain.
  • ドメインBoth DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
  • 細胞内局在Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGCRK6 antibody
    • C22orf12 antibody
    • D16H22S788E antibody
    • D16Wis2 antibody
    • DGCR 8 antibody
    • Dgcr8 antibody
    • DGCR8 microprocessor complex subunit antibody
    • DGCR8_HUMAN antibody
    • DGCRK 6 antibody
    • DiGeorge syndrome critical region 8 antibody
    • DiGeorge syndrome critical region gene 8 antibody
    • Gy1 antibody
    • Microprocessor complex subunit DGCR8 antibody
    • pasha antibody
    see all

Anti-DGCR8 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGCR8 with ab90579 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DGCR8 antibody (ab90579) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 86 kDa


    Exposure time : 3 minutes
  • Detection of Human DGCR8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab90579 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-DGCR8 antibody (ab90579) 使用論文

This product has been referenced in:
  • Slezak-Prochazka I  et al. Cellular localization and processing of primary transcripts of exonic microRNAs. PLoS One 8:e76647 (2013). IP . Read more (PubMed: 24073292) »
  • Caramuta S  et al. Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma. Blood Cancer J 3:e152 (2013). WB ; Human . Read more (PubMed: 24121164) »

See all 3 Publications for this product

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Thank you for your reply.

I have sent you a vial of ab36865 as a free of charge replacement for ab90579 which was not working for you in western blotting. Your new order number for your records only is***.

If you experience any issues...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab90579 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human (but not mouse) sample...

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