製品の概要

  • 製品名Anti-DGCR8 antibody
    DGCR8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DGCR8
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 180-229 (DKKDEENELD QEKRVEYAVL DELEDFTDNL ELDEEGAGGF TAKAIVQRDR) of human DGCR8 (NP_073557).

  • ポジティブ・コントロール
    • Hela cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab82876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 WW domain.
  • ドメインBoth DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
  • 細胞内局在Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGCRK6 antibody
    • C22orf12 antibody
    • D16H22S788E antibody
    • D16Wis2 antibody
    • DGCR 8 antibody
    • Dgcr8 antibody
    • DGCR8 microprocessor complex subunit antibody
    • DGCR8_HUMAN antibody
    • DGCRK 6 antibody
    • DiGeorge syndrome critical region 8 antibody
    • DiGeorge syndrome critical region gene 8 antibody
    • Gy1 antibody
    • Microprocessor complex subunit DGCR8 antibody
    • pasha antibody
    see all

Anti-DGCR8 antibody 画像

  • Anti-DGCR8 antibody (ab82876) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) ananlysis of human liver tissue labelling DGCR8 with ab82876 at 4-8µg/ml.

Anti-DGCR8 antibody (ab82876) 使用論文

This product has been referenced in:
  • Abu-Bonsrah KD  et al. CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities. Sci Rep 6:34524 (2016). Chicken . Read more (PubMed: 27694906) »
  • Van Duyne R  et al. Localization and sub-cellular shuttling of HTLV-1 tax with the miRNA machinery. PLoS One 7:e40662 (2012). WB ; Human . Read more (PubMed: 22808228) »

See all 3 Publications for this product

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