製品の概要

  • 製品名Anti-DGAT2 antibody
    DGAT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DGAT2
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Pig
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 153-362 of Human DGAT2 (NP_115953).

  • ポジティブ・コントロール
    • WB: Raji whole cell lysate IHC-P: A549 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • 組織特異性Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • パスウェイGlycerolipid metabolism; triacylglycerol biosynthesis.
  • 配列類似性Belongs to the diacylglycerol acyltransferase family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody 画像

  • Anti-DGAT2 antibody (ab96094) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 44 kDa
  • ab96094, at 1/100 dilution, staining DGAT2 in paraffin embedded A549 xenograft by Immunohistochemistry.
  • ICC/IF image of ab96094 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96094, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DGAT2 antibody (ab96094) 使用論文

This product has been referenced in:
  • Lee TI  et al. Cardiac metabolism, inflammation, and peroxisome proliferator-activated receptors modulated by 1,25-dihydroxyvitamin D3 in diabetic rats. Int J Cardiol 176:151-7 (2014). Rat . Read more (PubMed: 25062566) »
  • Jin J  et al. Increased expression of enzymes of triglyceride synthesis is essential for the development of hepatic steatosis. Cell Rep 3:831-43 (2013). IHC-P . Read more (PubMed: 23499441) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. I am sorry to read that your results were not as expected.

I will be happy to discuss a refund or make suggestions for a replacement but first, could you please send me a few details of the western blot samples, ...

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Thank you for your reply. You can go ahead and order the antibody, and redeem this code with your next order after submitting your Abreview for ab96094. DISCOUNT CODE: *** Expiration date: April 1, 2012 I am very pleased to hear you would like to test ...

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Thank you for your reply. Since ab96094 has not been tested in mouse or rat, I can issue you the testing code as soon as you are ready. All of the information that we have about this antibody is on the datasheet, so based on this information it seems t...

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Thank you for contacting us with your enquiry. We do have a testing discount program for trying an antibody in an untested species or application. We have tested ab96094 for immunofluorescent staining of human cell lines- will you be using the ...

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