製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DGAT2
  • 特異性
    This antibody is not expected to cross-react with other DGAT members.
  • アプリケーション
    適用あり: Sandwich ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KLEHPTQQDIDLYH

    , corresponding to C terminal amino acids 346-359 of Human DGAT2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

ターゲット情報

  • 機能
    Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • 組織特異性
    Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • パスウェイ
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • 配列類似性
    Belongs to the diacylglycerol acyltransferase family.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody 画像

  • ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

  • All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 3 : Tissue lysates prepared from human liver tissue.


    Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
  • Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg

Anti-DGAT2 antibody (ab59493) 使用論文

ab59493 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input
500 µg
Specification
Hepa1-6 hepatoma cells
Immuno-precipitation step
Protein A
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投稿 Sep 05 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount
40 µg
Specification
Hepa1-6 hepatoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Apr 17 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Loading amount
40 µg
Specification
Mouse muscle
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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