製品の概要

  • 製品名Anti-DGAT2 antibody
    DGAT2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to DGAT2
  • 特異性This antibody is not expected to cross-react with other DGAT members.
  • アプリケーション適用あり: Sandwich ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KLEHPTQQDIDLYH

    , corresponding to C terminal amino acids 346-359 of Human DGAT2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

ターゲット情報

  • 機能Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • 組織特異性Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • パスウェイGlycerolipid metabolism; triacylglycerol biosynthesis.
  • 配列類似性Belongs to the diacylglycerol acyltransferase family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody 画像

  • ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

  • All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 3 : Tissue lysates prepared from human liver tissue.


    Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
  • Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg

Anti-DGAT2 antibody (ab59493) 使用論文

ab59493 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input 500 µg
Specification Hepa1-6 hepatoma cells
Immuno-precipitation step Protein A
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投稿 Sep 05 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount 40 µg
Specification Hepa1-6 hepatoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Apr 17 2012

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse muscle)
Loading amount 40 µg
Specification Mouse muscle
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"