製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DFFB
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human DFFB.

    (Peptide available as ab89389.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; K562; HeLa; HeLa Nuclear.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • 配列類似性
    Contains 1 CIDE-N domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAD antibody
    • Caspase activated deoxyribonuclease antibody
    • Caspase activated DNase antibody
    • Caspase activated nuclease antibody
    • Caspase-activated deoxyribonuclease antibody
    • Caspase-activated DNase antibody
    • Caspase-activated nuclease antibody
    • CPAN antibody
    • Deoxyribonuclease III, caspase activated antibody
    • DFF 40 antibody
    • DFF-40 antibody
    • DFF2 antibody
    • DFF40 antibody
    • Dffb antibody
    • DFFB_HUMAN antibody
    • Didff antibody
    • DNA fragmentation factor 40 kDa subunit antibody
    • DNA fragmentation factor subunit beta antibody
    • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
    • DNA fragmentation factor, 40 kD beta subunit antibody
    • DNA fragmentation factor, 40 kD, beta polypeptide antibody
    • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
    • OTTHUMP00000003633 antibody
    see all

画像

  • All lanes : Anti-DFFB antibody (ab69438) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 15 kDa (possible isoform),30 kDa (possible isoform),75 kDa,77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab69438 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69438, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5µg/ml.

参考文献

ab69438 has not yet been referenced specifically in any publications.

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