Anti-Detyrosinated + Decarboxylated alpha Tubulin 抗体 [AA12] (ab24622)


  • 製品名Anti-Detyrosinated + Decarboxylated alpha Tubulin antibody [AA12]
  • 製品の詳細
    Mouse monoclonal [AA12] to Detyrosinated + Decarboxylated alpha Tubulin
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rabbit
    交差が予測される動物種: all Mammals
  • 免疫原

    This antibody was raised against the peptide sequence





  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名AA12
  • アイソタイプIgG2a
  • 研究分野


Our Abpromise guarantee covers the use of ab24622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21421796
WB 1/1000. Predicted molecular weight: 50 kDa.


  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 別名
    • Alpha-tubulin 1 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • Tuba4a antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    see all

Anti-Detyrosinated + Decarboxylated alpha Tubulin antibody [AA12] (ab24622) 使用論文

This product has been referenced in:
  • Nagai J  et al. Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation. Sci Rep 5:8269 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25652774) »
  • Akisaka T  et al. Differential distribution of posttranslationally modified microtubules in osteoclasts. J Histochem Cytochem 59:630-8 (2011). ICC/IF ; Rabbit . Read more (PubMed: 21421796) »

See all 3 Publications for this product

Product Wall

My apologies for the duplicate email, but I forgot to mention that I have added this code to your review and it is now active and ready to be used.

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Thank you very much for your email, and for your review of ab24622.

This code expired in August, which is probably the reason why it is not working at this time. Although we normally don't issue new codes past the expiration date, I've gone a...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (E18 primary hippocampal neurons)
Specification E18 primary hippocampal neurons
Fixative PHEM buffer (60 mM Pipes, 25 mM Hepes, 5 mM EGTA, and 1 mM MgCl) containing 0.25% glutaraldehyde, 3.7% paraformaldehyde, 3.7% sucrose, and 0.1% Triton X-100
Permeabilization Yes - Triton X-100 (in fixation solution)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

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Verified customer

投稿 Jun 20 2012

Thank you for your reply. I have issued the discount code below, and please let me know if you have any questions or if there is anything else that we can do for you.

Expiration date: August 18, 2012

This cod...

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Thank you for contacting us with your enquiry.

To our knowledge, ab24622 has not been tested in rat samples or in IP.So this antibody will quality for our testing discount program. In this program,youwould initally purchaseab24622, test it i...

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Expiration date: XX-XXX-XX

I am very pleased to hear you would like to accept our offer and test ab24622 in rat species with ICC/IF. This code will give you 1 free primary antibody before the expiration date. T...

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Thank you for contacting us.

This antibody has not been tested in rat species, but it’s very likely it will cross react with this species, due to sequence homology.

If you are interested in testing ab24622 in rat samples with ...

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