Anti-Detyrosinated alpha Tubulin 抗体 (ab48389)

製品の概要

  • 製品名
    Anti-Detyrosinated alpha Tubulin antibody
  • 製品の詳細
    Rabbit polyclonal to Detyrosinated alpha Tubulin
  • 特異性
    This product reacts specifically with human Detyrosinated alpha Tubulin.
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.

  • 特記事項

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500.
WB 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559).

ターゲット情報

  • 関連性
    Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • Alpha tubulin 1 antibody
    • TUBA1 antibody
    • TUBA2 antibody
    • TUBA3C antibody
    • TUBA4A antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 1 testis specific antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    see all

画像

  • All lanes : Anti-Detyrosinated alpha Tubulin antibody (ab48389) at 1/500 dilution

    Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minuteDetyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.

参考文献

This product has been referenced in:
  • Verdeny-Vilanova I  et al. 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells. J Cell Sci 130:1904-1916 (2017). Read more (PubMed: 28420672) »
  • Gao Y  et al. Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2. Sci Rep 7:1110 (2017). WB, ICC/IF ; Rat . Read more (PubMed: 28439067) »

See all 15 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (MTLn3 cells)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
140 µg
Specification
MTLn3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Neha Kaul

Verified customer

投稿 Sep 15 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (Swine testicle cells (ST))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
35 µg
Treatment
mock and GFP transfection
Specification
Swine testicle cells (ST)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Robert Jansens

Verified customer

投稿 Mar 02 2017

Application
IHC - Wholemount
Sample
Zebrafish Embryo (whole embryos caudal fin imaged)
Specification
whole embryos caudal fin imaged
Username

Abcam user community

Verified customer

投稿 May 12 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (swine testicle (ST))
Permeabilization
Yes - triton
Specification
swine testicle (ST)
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 11 2015

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Sertoli cells)
Permeabilization
Yes - Triton
Specification
Sertoli cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Methanol
Username

Dr. Nan Li

Verified customer

投稿 Sep 09 2015

Application
Western blot
Sample
Rat Cell lysate - whole cell (Sertoli cells)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
20 µg
Specification
Sertoli cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Nan Li

Verified customer

投稿 Sep 09 2015

Thank you for your enquiry regarding ab48389.

This product is whole serum and not affinity purified so the concentration is not determined. You can find some more information about the concentration of the different purifications at this site...

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Thank you for confirming these details and for your cooperation. Your enquiry has been forwarded to me as my colleague Cathrin is currently away from the office.

The details provided enable us to closely monitor the quality of our products. ...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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