製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Destrin
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the internal sequence amino acids of Human Destrin (NP_006861.1).

  • ポジティブ・コントロール
    • MCF-7 cell line lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
  • 組織特異性
    Widely distributed in various tissues.
  • 配列類似性
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻訳後修飾
    ISGylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610043P17Rik antibody
    • ACTDP antibody
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AU042046 antibody
    • bA462D18.2 antibody
    • corn1 antibody
    • DEST_HUMAN antibody
    • Destrin (actin depolymerizing factor) antibody
    • Destrin antibody
    • DSN antibody
    • Dstn antibody
    • Epididymis luminal protein 32 antibody
    • HEL32 antibody
    • Sid 23 antibody
    • sid23p antibody
    see all

画像

  • Anti-Destrin antibody (ab95298) at 1/100 dilution + MCF-7 cell line lysate at 35 µg

    Predicted band size : 19 kDa
  • IHC-P image of Destrin (ab95298) on Human Kidney sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in H2O2 solution for 10 mins at 22°C.

    See Abreview

参考文献

This product has been referenced in:
  • Zhang YY  et al. Proteomics reveals potential non-neuronal cholinergic receptor-effectors in endothelial cells. Acta Pharmacol Sin 35:1137-49 (2014). Read more (PubMed: 25088000) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization
No
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3µg/mL · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

投稿 Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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