製品の概要

  • 製品名Anti-Desmuslin antibody [M03]
    Desmuslin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M03] to Desmuslin
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 1466-1565 (VSNVEAIRSR TQEAGALGVS DRGSWRDADS RNDQAVGVSF KASAGEGDQA HREQGKEQAM FDKKVQLQRM VDQRSVISDE KKVALLYLDN EEEENDGHWF) of human Desmuslin (NP_663780) linked to a proprietary tag.

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項

    The recombinant fragment used as the immunogen is available as ab161606.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名M03
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab89228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 173 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
  • 組織特異性Isoform 2 is strongly detected in adult heart, fetal skeletal muscles and fetal heart. Isoform 1 is weakly detected in fetal heart and also in fetal skeletal muscle. Isoform 1 and isoform 2 are detected in adult bladder (at protein level). The mRNA is predominantly expressed in heart and muscle with some expression in brain which may be due to tissue-specific isoforms.
  • 配列類似性Belongs to the intermediate filament family.
  • 発生段階In lens, first detected at 16 weeks when expression is weakly and uniformly distributed. Subsequently, expression becomes much stronger in the epithelium of the anterior part at 25 weeks and later. In retina, weakly expressed at 15 weeks in the nerve fiber and ganglion cell layers (NFL and GCL). From 25 weeks onwards, much stronger expression is observed in the endfeet of Mueller cells, the NFL, and GCL, and much lower expression is observed in a minor subpopulation of cells in the inner cell layer (INL). At 30 and 36 weeks, expression remains in the neural retina, and subsequently becomes stronger in the NFL, GCL, and INL and is decreased in Mueller cells. At 36 weeks, also expressed at the external border of the outer nuclear layer (ONL) (at protein level).
  • 細胞内局在Cytoplasm, cytoskeleton. Cell junction, adherens junction. There are at least two distinct SYNM subpopulations, one in which SYMN interacts with DES within the Z-lines, and another in which it interacts with both DTNA and DES at the costamere.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Desmuslin antibody
    • DMN antibody
    • KIAA0353 antibody
    • SYN antibody
    • SYNEM_HUMAN antibody
    • Synemin alpha antibody
    • Synemin antibody
    • Synemin beta antibody
    • Synemin, intermediate filament protein antibody
    • SYNM antibody
    see all

Anti-Desmuslin antibody [M03] 画像

  • Anti-Desmuslin antibody [M03] (ab89228) at 5 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 173 kDa
    Observed band size : 173 kDa

Anti-Desmuslin antibody [M03] (ab89228) 使用論文

ab89228 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"