製品の概要

  • 製品名Anti-Desmoplakin I+II antibody [DP2.15]
    Desmoplakin I+II 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DP2.15] to Desmoplakin I+II
  • アプリケーション適用あり: WB, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Full length native protein (purified)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.5% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名DP2.15
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab35577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-Fr
ICC/IF
  • 追加情報ICC/IF: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 319 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
    • 組織特異性Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
    • 関連疾患Keratoderma, palmoplantar, striate 2
      Cardiomyopathy, dilated, with woolly hair and keratoderma
      Arrhythmogenic right ventricular dysplasia, familial, 8
      Skin fragility-woolly hair syndrome
      Epidermolysis bullosa, lethal acantholytic
      Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
    • 配列類似性Belongs to the plakin or cytolinker family.
      Contains 17 plectin repeats.
      Contains 1 SH3 domain.
      Contains 6 spectrin repeats.
    • ドメインIts association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
      The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
      The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
    • 翻訳後修飾Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
      Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
    • 細胞内局在Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
    • Information by UniProt
    • 参照データベース
    • 別名
      • 250/210 kDa paraneoplastic pemphigus antigen antibody
      • DCWHKTA antibody
      • Desmoplakin (DPI DPII) antibody
      • Desmoplakin antibody
      • Desmoplakin I antibody
      • Desmoplakin II antibody
      • DESP_HUMAN antibody
      • DP antibody
      • DP I antibody
      • DP II antibody
      • DPI antibody
      • DPII antibody
      • DSP antibody
      • KPPS2 antibody
      • PPKS 2 antibody
      • PPKS2 antibody
      see all

    Anti-Desmoplakin I+II antibody [DP2.15] (ab35577) 使用論文

    This product has been referenced in:
    • Werner NS  et al. Epidermolysis bullosa simplex-type mutations alter the dynamics of the keratin cytoskeleton and reveal a contribution of actin to the transport of keratin subunits. Mol Biol Cell 15:990-1002 (2004). Read more (PubMed: 14668478) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"