製品の概要

  • 製品名
    Anti-Desmoplakin I+II antibody
    Desmoplakin I+II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Desmoplakin I+II
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human Desmoplakin I+II.

    (Peptide available as ab71689.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Caco 2 whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238, 270 kDa (predicted molecular weight: 260 , 332 kDa).Can be blocked with Human Desmoplakin I+II peptide (ab71689).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
  • 組織特異性
    Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
  • 関連疾患
    Keratoderma, palmoplantar, striate 2
    Cardiomyopathy, dilated, with woolly hair and keratoderma
    Arrhythmogenic right ventricular dysplasia, familial, 8
    Skin fragility-woolly hair syndrome
    Epidermolysis bullosa, lethal acantholytic
    Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
  • 配列類似性
    Belongs to the plakin or cytolinker family.
    Contains 17 plectin repeats.
    Contains 1 SH3 domain.
    Contains 6 spectrin repeats.
  • ドメイン
    Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
    The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
    The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
  • 翻訳後修飾
    Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
  • 細胞内局在
    Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • 250/210 kDa paraneoplastic pemphigus antigen antibody
    • DCWHKTA antibody
    • Desmoplakin (DPI DPII) antibody
    • Desmoplakin antibody
    • Desmoplakin I antibody
    • Desmoplakin II antibody
    • DESP_HUMAN antibody
    • DP antibody
    • DP I antibody
    • DP II antibody
    • DPI antibody
    • DPII antibody
    • DSP antibody
    • KPPS2 antibody
    • PPKS 2 antibody
    • PPKS2 antibody
    see all

Anti-Desmoplakin I+II antibody 画像

  • Desmoplakin I+II antibody (ab71690) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 260 , 332 kDa
    Observed band size : 238,270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,45 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71690 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71690, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.

Anti-Desmoplakin I+II antibody (ab71690) 使用論文

This product has been referenced in:
  • Galoian K  et al. Restoration of desmosomal junction protein expression and inhibition of H3K9-specific histone demethylase activity by cytostatic proline-rich polypeptide-1 leads to suppression of tumorigenic potential in human chondrosarcoma cells. Mol Clin Oncol 3:171-178 (2015). Human . Read more (PubMed: 25469290) »
  • Wang X  et al. Synergy Analysis Reveals Association between Insulin Signaling and Desmoplakin Expression in Palmitate Treated HepG2 Cells. PLoS One 6:e28138 (2011). ICC/IF ; Human . Read more (PubMed: 22132232) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuronal)
Specification
neuronal
Fixative
Methanol
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: rt°C
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Abcam user community

Verified customer

投稿 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic kidney cells hek293)
Loading amount
40 µg
Specification
human embryonic kidney cells hek293
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

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Verified customer

投稿 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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