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Full length protein (Cow).
Our Abpromise guarantee covers the use of ab16434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 331 kDa.|
ab16434 staining Desmoplakin I+II in rat glial cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% serum) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.