製品の概要

  • 製品名Anti-Desmoplakin antibody [EPR4383(2)]
    Desmoplakin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4383(2)] to Desmoplakin
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Desmoplakin aa 400-500. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: A431, HACAT and BXPC-3 cell lysates. ICC/IF: A431 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Desmoplakin antibody (Alexa Fluor® 647) [EPR4383(2)] (ab207531)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 332 kDa.
ICC/IF 1/1000.

ターゲット情報

  • 関連性Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin.
  • 細胞内局在Cell junction, desmosome. Cytoplasm, cytoskeleton.
  • 参照データベース
  • 別名
    • 250/210 kDa paraneoplastic pemphigus antigen antibody
    • Desmoplakin I antibody
    • Desmoplakin II antibody
    • DP I antibody
    • DP II antibody
    • DPI antibody
    • DPII antibody
    • DSP antibody
    • KPPS2 antibody
    • PPKS2 antibody
    see all

Anti-Desmoplakin antibody [EPR4383(2)] 画像

  • Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : BXPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 332 kDa

Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) 使用論文

This product has been referenced in:
  • Hartlieb E  et al. Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes. J Biol Chem 289:17043-53 (2014). WB . Read more (PubMed: 24782306) »
  • Hartlieb E  et al. Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion. PLoS One 8:e53739 (2013). WB ; Human . Read more (PubMed: 23326495) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (BioRad 4-15% TGX gel)
Sample Mouse Cell lysate - whole cell (Mammary Organoids)
Specification Mammary Organoids
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Mrs. Amanda Fairchild

Verified customer

投稿 May 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"