Anti-Desmoglein 1 抗体 [EPR6766(B)] (ab124798)

製品の概要

  • 製品名Anti-Desmoglein 1 antibody [EPR6766(B)]
    Desmoglein 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6766(B)] to Desmoglein 1
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C-terminal of Human Desmoglein 1 (Q02413).

  • ポジティブ・コントロール
    • Human skin lysate, Human skin tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • 組織特異性Epidermis, tongue, tonsil and esophagus.
    • 関連疾患Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
    • 配列類似性Contains 4 cadherin domains.
    • ドメインCalcium may be bound by the cadherin-like repeats.
    • 細胞内局在Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    Anti-Desmoglein 1 antibody [EPR6766(B)] 画像

    • Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 114 kDa
    • ab124798, at a 1/250 dilution, staining Desmoglein 1 in paraffin embedded Human skin tissue by Immunohistochemistry.

    Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798) 使用論文

    ab124798 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (skin)
    Specification skin
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Mar 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample Mouse Tissue sections (epidermis)
    Specification epidermis
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 29 2013

    Abreviews
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Mouse Tissue sections (Epidermis)
    Specification Epidermis
    Permeabilization No
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    投稿 May 29 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (epidermis)
    Specification epidermis
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 May 29 2013

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"