製品の概要

  • 製品名Anti-Desmoglein 1 antibody [32-2B]
    Desmoglein 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [32-2B] to Desmoglein 1
  • 特異性This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Bovine desmoglein (desmosomal glycoprotein I) from nasal epidermis.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • 組織特異性Epidermis, tongue, tonsil and esophagus.
  • 関連疾患Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • 配列類似性Contains 4 cadherin domains.
  • ドメインCalcium may be bound by the cadherin-like repeats.
  • 細胞内局在Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all

Anti-Desmoglein 1 antibody [32-2B] 画像

  • IHC image of Desmoglein 1 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Desmoglein 1 antibody [32-2B] (ab108490) 使用論文

This product has been referenced in:
  • Conn IG  et al. Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas. Br J Urol 65:176-80 (1990). Read more (PubMed: 2317652) »
  • Vilela MJ  et al. Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology. J Pathol 153:365-75 (1987). Read more (PubMed: 3323436) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Human Cell (Keratinocytes)
Specification Keratinocytes
Permeabilization Yes - Methanol
Fixative Methanol
Username

Ms. Sabine Duchatelet

Verified customer

投稿 Mar 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"