製品の概要

  • 製品名
    Anti-Desmoglein 1 antibody [27B2]
    Desmoglein 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [27B2] to Desmoglein 1
  • 特異性
    Ab12077 reacts with Desmoglein 1. Does not cross-react with Desmoglein 2 or Desmoglein 3.
  • アプリケーション
    適用あり: IHC-P, WB, IP, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Unfortunately, this information is considered to be commercially sensitive

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10. Predicted molecular weight: 165 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • 組織特異性
    Epidermis, tongue, tonsil and esophagus.
  • 関連疾患
    Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • 配列類似性
    Contains 4 cadherin domains.
  • ドメイン
    Calcium may be bound by the cadherin-like repeats.
  • 細胞内局在
    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all

画像

  • ab12077 staining Desmoglein 1 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Tsutsumi M  et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . Read more (PubMed: 19657674) »
  • Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . Read more (PubMed: 17971852) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil epithelium)
Specification
Tonsil epithelium
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4/ 1mMEDTA
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Methanol
Permeabilization
Yes - 0.4% TritonX-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 08 2011

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab10277. To check the status of the order please contact our Customer...

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Thank you for your enquiry. According to the NCBI-protein site aa 664-763 are located in the cytoplasmic region of the protein. The cytoplasmic staining is therefore correct. I hope this information helps, please do not hesitate to contact us if you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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