Anti-Desmocollin 3 抗体 (ab55011)

製品の概要

  • 製品名Anti-Desmocollin 3 antibody
    Desmocollin 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Desmocollin 3
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: CKPKMGYTDI LAVDPDEPVH GAPFYFSLPN TSPEISRLWS LTKVNDTAAR LSYQKNAGFQ EYTIPITVKD RAGQAATKLL RVNLCECTHP TQCRATSRST , corresponding to amino acids 585-685 of Human Desmocollin 3

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 100 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
  • 組織特異性Epidermis, buccal mucosa, esophagus and cervix.
  • 関連疾患Defects in DSC3 are the cause of hypotrichosis and recurrent skin vesicles (HRSV) [MIM:613102]. A disorder characterized by hypotrichosis and the appearance of recurrent skin vesicle formation. Affected individuals show sparse and fragile hair on scalp, as well as absent eyebrows and eyelashes. Vesicles filled with thin, watery fluid are observed on the scalp and skin of most of the boby. Mucosal vesicles are absent.
  • 配列類似性Contains 5 cadherin domains.
  • ドメインCalcium may be bound by the cadherin-like repeats.
  • 細胞内局在Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cadherin family member 3 antibody
    • CDHF3 antibody
    • desmocollin 3a/3b antibody
    • Desmocollin 4 antibody
    • Desmocollin-3 antibody
    • Desmocollin-4 antibody
    • Desmocollin3 antibody
    • Desmocollin4 antibody
    • DSC antibody
    • DSC1 antibody
    • DSC2 antibody
    • DSC3 antibody
    • DSC3_HUMAN antibody
    • DSC4 antibody
    • HT CP antibody
    • HT-CP antibody
    • HTCP antibody
    see all

Anti-Desmocollin 3 antibody 画像

  • Overlay histogram showing A431 cells stained with ab55011 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55011, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab55011 at 10 ug/ml staining Desmocollin 3 in human A-431 cells by Immunocytochemistry/ Immunofluorescence.

Anti-Desmocollin 3 antibody (ab55011) 使用論文

ab55011 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

I am pleased to provide the following information:

The immunogen sequence for this antibody (ab55011Desmocollin 3)is provided on the datasheet:

'Recombinant fragment: CKPKMGYTDI LAVDPDEPVH GA...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"