製品の概要
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製品名Anti-DENND1B antibody
DENND1B 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DENND1B
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Zebrafish
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免疫原
Synthetic peptide: PVNLSVNQEI FIACEQVLKD QPALVPHSYF IAPDVTGLPT IPESRNLTEY , corresponding to a region within internal sequence amino acids 144 - 193 of Human DENND1B isoform 1 (NP_659414)
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ポジティブ･コントロール
- 293T cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab84347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
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組織特異性Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.
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配列類似性Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain. -
翻訳後修飾Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.
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細胞内局在Cytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle.
- Information by UniProt
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参照データベース
- Entrez Gene: 515939 Cow
- Entrez Gene: 100061134 Horse
- Entrez Gene: 163486 Human
- Entrez Gene: 329260 Mouse
- Entrez Gene: 100338926 Rabbit
- Entrez Gene: 289051 Rat
- Omim: 613292 Human
- SwissProt: Q6P3S1 Human
see all -
別名
- C1ORF18 antibody
- C1orf218 antibody
- Chromosome 1 open reading frame 218 antibody
see all
Anti-DENND1B antibody 画像
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Western blot - DENND1B antibody (ab84347)Anti-DENND1B antibody (ab84347) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 48 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
プロトコール
Anti-DENND1B antibody (ab84347) 使用論文
ab84347 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"