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Full length native protein purified from Dengue Virus 2 (16681) infected supernatant
Our Abpromise guarantee covers the use of ab41623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/80. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/5 - 1/20.|
ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ab41623 staining Dengue Virus NS1 glycoprotein in HeLa cells at 62hpi with DV2 by Flow Cytometry. Cells were fixed and permeabilized with BD CytoFix/CytoPerm. The sample was incubated with the primary antibody (1/80 in CytoFix/CytoPerm) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/150) was used as the secondary antibody.
Gating Strategy: Single cells by FSC and SSC.