Anti-Dengue Virus NS1 glycoprotein 抗体 [DN2] (ab41623)
Key features and details
- Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Dengue virus 2
- Isotype: IgG1
製品の概要
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製品名
Anti-Dengue Virus NS1 glycoprotein antibody [DN2]
Dengue Virus NS1 glycoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [DN2] to Dengue Virus NS1 glycoprotein -
由来種
Mouse -
特異性
Recognises NS1 from both Dengue Virus 2 strains, 16681 and NGC. -
アプリケーション
適用あり: Flow Cyt, WB, ICC/IFmore details -
種交差性
交差種: Dengue virus 2 -
免疫原
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
DN2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41623の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB |
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa.
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ICC/IF | (1) |
1/5 - 1/20.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 40 kDa. |
ICC/IF
1/5 - 1/20. |
ターゲット情報
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関連性
NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever. -
参照データベース
- SwissProt: P29990 Dengue virus 2
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別名
- Dengue NS1 antibody
- Dengue Virus non structural protein 1 antibody
画像
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ab41623 staining Dengue Virus NS1 glycoprotein (green) in Human BHK cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed and permeabilized with CytoFix/CytoPerm and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/20) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN2] (ab41623)
Lane 1 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (unheated)
Lane 2 : Dengue Virus 2 (NGC)infected C6/36 cell lysate (boiled)
Lane 3 : Dengue Virus 2 (16681)infected C6/36 cell lysate (unheated)
Lane 4 : Dengue Virus 2 (16681)infected C6/36 cell lysate (boiled)
Predicted band size: 40 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
NS1 exists as a dimer (~80 kDa) in unheated samples but is dissociated into a monomer (~40 kDa) when samples are boiled. Ab41623 recognised both forms of NS1.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab41623 は 7 報の論文で使用されています。
- Labeau A et al. A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection. J Virol 94:N/A (2020). PubMed: 31915280
- Yao X et al. Tatanan A from the Acorus calamus L. root inhibited dengue virus proliferation and infections. Phytomedicine 42:258-267 (2018). WB . PubMed: 29655694
- Durbin AF et al. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio 7:N/A (2016). PubMed: 27651356
- Scaturro P et al. Dengue Virus Non-structural Protein 1 Modulates Infectious Particle Production via Interaction with the Structural Proteins. PLoS Pathog 11:e1005277 (2015). PubMed: 26562291
- Immenschuh S et al. Antibodies against dengue virus nonstructural protein-1 induce heme oxygenase-1 via a redox-dependent pathway in Human endothelial cells. Free Radic Biol Med : (2012). PubMed: 23103292
- Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF . PubMed: 21658241
- Tong XK et al. WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro. Acta Pharmacol Sin 31:585-92 (2010). Flow Cyt . PubMed: 20418898