製品の概要

  • 製品名Anti-DeltaD antibody [Dld 5C7/2, zdd2]
    DeltaD 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD
  • 特異性This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.
  • アプリケーション適用あり: ELISA, WB, IHC-Fr, Flow Cyt, ICC/IF, IHC - Wholemountmore details
  • 種交差性
    交差種: Zebrafish
  • 免疫原

    Extracellular DeltaD peptide (Zebrafish).

  • エピトープZebrafish DeltaD protein extracellular domain.
  • ポジティブ・コントロール
    • HEK293 cells transfected with deltaD or zebrafish embryo.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.

ターゲット情報

  • 関連性DeltaD is one of the 4 members of the zebrafish Delta family of Notch ligands, important in the control of cell differentiation in many tissues. DeltaD acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. It can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized.
  • 細胞内局在Cell Membrane
  • 参照データベース
    • 別名
      • Aei antibody
      • After eight antibody
      • Dld antibody

    Anti-DeltaD antibody [Dld 5C7/2, zdd2] 画像

    • ab73331 staining DeltaD in wild-type (upper panel) and ktoy82 (lower panel) zebrafish embryo hindbrain, by Immunohistochemistry - Wholemount.

    Anti-DeltaD antibody [Dld 5C7/2, zdd2] (ab73331) 使用論文

    This product has been referenced in:
    • Rothschild SC  et al. CaMK-II activation is essential for zebrafish inner ear development and acts through Delta-Notch signaling. Dev Biol 381:179-88 (2013). Read more (PubMed: 23747599) »
    • Hong SK & Dawid IB The transcriptional mediator component med12 is required for hindbrain boundary formation. PLoS One 6:e19076 (2011). IHC - Wholemount ; Zebrafish . Read more (PubMed: 21533047) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Zebrafish Tissue sections (whole embryo)
    Specification whole embryo
    Username

    Ms. Emerald Butko

    Verified customer

    投稿 Oct 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"