製品の概要

  • 製品名
    Anti-delta II tubulin antibody
  • 製品の詳細
    Rabbit polyclonal to delta II tubulin
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human delta II tubulin (C terminal). 10 amino acid synthetic peptide.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab106658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 24022482
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

画像

  • Figure to show Immunostaining of CCP5 enzymatic activity toward polymerized tubulin. Polymerized microtubules from MDA-MB-231 cells were prepared on plates using microtubule-stabilizing buffer containing 5 μm paclitaxel in the absence of GTP. For immunostaining with tyrosinated tubulin (ab48389) and Δ2-tubulin antibodies (ab106658), microtubules were treated with carboxypeptidase A1 (40 ng/ml) to convert tyrosinated into detyrosinated tubulin. After washes, microtubules were incubated with CCP5 or control eluate for 2 h and then processed for immunocytochemistry.

参考文献

This product has been referenced in:
  • Berezniuk I  et al. Cytosolic Carboxypeptidase 5 Removes a- and ?-Linked Glutamates from Tubulin. J Biol Chem 288:30445-53 (2013). ICC/IF ; Human . Read more (PubMed: 24022482) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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