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Synthetic peptide corresponding to Human delta II tubulin (C terminal). 10 amino acid synthetic peptide.
Our Abpromise guarantee covers the use of ab106658 in the following tested applications.
|WB||1/500 - 1/2000.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24022482|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Figure to show Immunostaining of CCP5 enzymatic activity toward polymerized tubulin. Polymerized microtubules from MDA-MB-231 cells were prepared on plates using microtubule-stabilizing buffer containing 5 μm paclitaxel in the absence of GTP. For immunostaining with tyrosinated tubulin (ab48389) and Δ2-tubulin antibodies (ab106658), microtubules were treated with carboxypeptidase A1 (40 ng/ml) to convert tyrosinated into detyrosinated tubulin. After washes, microtubules were incubated with CCP5 or control eluate for 2 h and then processed for immunocytochemistry.