Anti-delta 1 Catenin/CAS (phospho Y228) 抗体 [E205] (ab32403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205]
delta 1 Catenin/CAS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y228).
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ポジティブ・コントロール
- WB: HeLa whole cell lysate (ab150035). IHC-P: Breast carcinoma.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E205 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32403の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
組織特異性
Expressed in vascular endothelium. -
配列類似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
ドメイン
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻訳後修飾
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Entrez Gene: 311163 Rat
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
- Unigene: 35738 Mouse
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別名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
画像
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All lanes : Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Pervanandate
Lane 3 : HeLa cell lysate, treated with Phosphatase
Predicted band size: 108 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted? -
Ab32403, at a 1/50 dilution, staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab32403 は 3 報の論文で使用されています。
- Park SY et al. Glycoprotein 90K Promotes E-Cadherin Degradation in a Cell Density-Dependent Manner via Dissociation of E-Cadherin-p120-Catenin Complex. Int J Mol Sci 18:N/A (2017). PubMed: 29207493
- Surana R et al. IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model. Cancer Immunol Res 2:1103-12 (2014). WB . PubMed: 25204776
- Ma LW et al. Phosphorylated p120-catenin expression has predictive value for oral cancer progression. J Clin Pathol 65:315-9 (2012). PubMed: 22259181