Anti-delta 1 Catenin (phospho T916) 抗体 [EPR2383] (ab79400)
製品の概要
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製品名Anti-delta 1 Catenin (phospho T916) antibody [EPR2383]
delta 1 Catenin 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2383] to delta 1 Catenin (phospho T916)
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由来種Rabbit
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特異性ab79400 only detects delta 1 Catenin phosphorylated on threonine 916.
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アプリケーション適用あり: WB, IHC-P, ICCmore details
適用なし: Flow Cyt or IP -
種交差性交差種: Human
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免疫原
A phospho-specific peptide corresponding to residues surrounding threonine 916 of human delta 1 Catenin
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ポジティブ･コントロール
- 293T cell lysates treated with calyculin A; human ovarian carcinoma tissue.
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2383
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab79400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC |
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/5000 - 1/50000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
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組織特異性Expressed in vascular endothelium.
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配列類似性Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
ドメインA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
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翻訳後修飾Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
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細胞内局在Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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別名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
画像
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![Western blot - Anti-delta 1 Catenin (phospho T916) antibody [EPR2383] (ab79400)](http://a.abcam.com/ps/products/79/ab79400/Images/delta-1-Catenin-Primary-antibodies-ab79400-1.jpg)
Western blot - Anti-delta 1 Catenin (phospho T916) antibody [EPR2383] (ab79400)All lanes : Anti-delta 1 Catenin (phospho T916) antibody [EPR2383] (ab79400) at 1/50000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 100 kDa (possible isoform) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin (phospho T916) antibody [EPR2383] (ab79400)](http://a.abcam.com/ps/products/79/ab79400/Images/delta-1-Catenin-Primary-antibodies-ab79400-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin (phospho T916) antibody [EPR2383] (ab79400)ab79400 at 1/100 dilution staining delta 1 Catenin in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
プロトコール
参考文献
ab79400 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"