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RabMAb

Anti-delta 1 Catenin (phospho S268) 抗体 [EPR2380] (ab79545)

製品の概要

  • 製品名
    Anti-delta 1 Catenin (phospho S268) antibody [EPR2380]
    delta 1 Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2380] to delta 1 Catenin (phospho S268)
  • 特異性
    ab79545 only detects delta 1 Catenin phosphorylated on serine 268.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding serine 268 of Human delta 1 Catenin.

  • ポジティブ・コントロール
    • HeLa cell lysate treated with Calyculin A. A431 cells. Human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • 組織特異性
      Expressed in vascular endothelium.
    • 配列類似性
      Belongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • ドメイン
      A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • 翻訳後修飾
      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • 細胞内局在
      Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    画像

    • Immunofluorescent staining of delta 1 Catenin in A431 cells using ab79545 at 1/100 dilution.
    • ab79545 at 1/100 dilution staining delta 1 Catenin in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-delta 1 Catenin (phospho S268) antibody [EPR2380] (ab79545) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 108 kDa
      Observed band size : 90-120 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab79545 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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