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RabMAb

Anti-delta 1 Catenin 抗体 [EPR357(2)] (ab92514)

製品の概要

  • 製品名
    Anti-delta 1 Catenin antibody [EPR357(2)]
    delta 1 Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR357(2)] to delta 1 Catenin
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human delta 1 Catenin

  • ポジティブ・コントロール
    • WB: HeLa, 293T and A431 cell lysates. IHC-P: Human breast carcinoma and colonic adenocarcinoma tissues. ICC/IF: A431 cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.

ICC/IF 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • 組織特異性
    Expressed in vascular endothelium.
  • 配列類似性
    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • ドメイン
    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • 翻訳後修飾
    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • 細胞内局在
    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Anti-delta 1 Catenin antibody [EPR357(2)] 画像

  • Immunofluorescence of A431 cells labelled with ab92514 at 1/100 dilution.
  • All lanes : Anti-delta 1 Catenin antibody [EPR357(2)] (ab92514) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 108 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Paraffin embedded Human breast carcinoma tissue (1) and Human colonic adenocarcinoma tissue (2) labelled with ab29514 at 1/100 dilution.
  • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin with unpurified ab92514 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-delta 1 Catenin antibody [EPR357(2)] (ab92514) 使用論文

This product has been referenced in:
  • Xiong Y  et al. Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma. Oncotarget 8:24902-24914 (2017). Human . Read more (PubMed: 28212546) »
  • Hu Y  et al. Melatonin protects against blood-brain barrier damage by inhibiting the TLR4/ NF-?B signaling pathway after LPS treatment in neonatal rats. Oncotarget 8:31638-31654 (2017). Read more (PubMed: 28404943) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
Undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
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Verified customer

投稿 Feb 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
HUES7 ES cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Feb 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Miss. Rebecca Atkinson-Dell

Verified customer

投稿 Nov 18 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Nov 13 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (hES and cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hES and cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Aug 20 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (lung adenocarcinoma mouse model tissue biopsy)
Specification
lung adenocarcinoma mouse model tissue biopsy
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jul 25 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (lung cancer line A549 and H23)
Specification
lung cancer line A549 and H23
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jul 25 2014

Application
Flow Cytometry
Fixation
Methanol
Permeabilization
Yes - 0.5% v/v tween 20 in PBS
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 5% fish skin gelatin
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投稿 Apr 30 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol:acetone 1:1
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投稿 Dec 20 2013

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

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投稿 Dec 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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