Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol:acetone 1:1
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/100
Additional data
Additional Notes
Blue is DAPI, green is ab92514. I also tried 4% PFA as a fixative but the staining did not work.
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投稿 Dec 20 2013