製品の概要

  • 製品名Anti-DEK antibody
    DEK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DEK
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    9aa synthetic peptide from the region between aa 178-198 of Human DEK

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーStabilization buffer
    Preservative: 0.02% Sodium Azide
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 43 kDa.

ターゲット情報

  • 機能Involved in chromatin organization.
  • 組織特異性Ubiquitous. Expressed at relatively high levels.
  • 関連疾患A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
  • 配列類似性Contains 1 SAP domain.
  • 翻訳後修飾Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
  • 細胞内局在Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D6S231E antibody
    • Dek antibody
    • DEK gene antibody
    • DEK oncogene antibody
    • DEK oncogene DNA binding antibody
    • DEK_HUMAN antibody
    • Protein DEK antibody
    see all

Anti-DEK antibody (ab74975) 使用論文

This product has been referenced in:
  • Sandén C  et al. The DEK oncoprotein binds to highly and ubiquitously expressed genes with a dual role in their transcriptional regulation. Mol Cancer 13:215 (2014). CHIPseq ; Human . Read more (PubMed: 25216995) »

See 1 Publication for this product

Product Wall

According to our records, ab74975 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

Read More

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
We had so far o...

Read More
Abreviews
Application ChIP
Sample Human Cell lysate - nuclear (Leukemia cell line)
Specification Leukemia cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Other
Positive control Nuclear lysate of untreated U937 cells.
Username

Abcam user community

Verified customer

投稿 Jan 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (testis)
Specification testis
Fixative Formaldehyde
Permeabilization Yes - Triton X-100 0,5%
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 16 2012

Thank you very much for your interest in ab74975. To our knowledge, ab74975 has not been tested in ChIP. I can also offer a discount off a future purchase if you buy ab74975 now, test it in ChIP and submit feedback to us in the form of an Abreview. It ...

Read More

Merci pour ces précisions. En effet, l'anti-DEK ab83099 est également un bon alternatif. Le numéro de commande de remplacement gratuit (une unité de ab83099) est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'ex...

Read More

Je parle effectivement français. Je suis désolé d'apprendre que les 2 anti-DEK ab22826 et ab76975 que vous avez testés ne fonctionnent pas comme indiqué sur les fiches techniques respectives. Votre protocole me semble bon, je crains que vous ayez r...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spe...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (knees - sections prepared from the whole knee)
Specification knees - sections prepared from the whole knee
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X-100
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"