Determined by RIA (3H): Dehydroepiandrosterone 100% DHEAS 2.6% Androsterone 0.28% Androstenedione 0.17% 20-Dihydroprogesterone 0.02% 11-Hydroxyprogesterone 0.02%. All other steroids tested, less than 0.002%.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/150 - 1/650. This antibody was tested in a simple ELISA (using 2 ug/ml of DHEA-7CMO-BSA coated to the plate), and gave a 50% binding titre of 1/360 and an endpoint titre of 1/100K.
Use at an assay dependent dilution.
1/100 - 1/300. If using an iodinated tracer, antibody can be diluted 5-10 times further.
Dehydroepiandrosterone (DHEA) is a major secretory product of the human adrenal cortex during intrauterine development as well as during adulthood. DHEA circulates in the bloodstream as DHEA-sulfate (DHEAS). DHEA has been implicated in a wide range of physiological roles including aging, immunology, learning and memory and obesity.