製品の概要

  • 製品名Anti-DEF6 antibody
    DEF6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DEF6
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-337 of Human DEF6

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
    • 組織特異性Broadly expressed in the immune system and can be detected in T and B-cells.
    • 配列類似性Contains 1 PH domain.
    • ドメインThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • 翻訳後修飾Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
    • 細胞内局在Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DEF-6 antibody
      • Def6 antibody
      • DEFI6_HUMAN antibody
      • Differentially expressed in FDCP 6 homolog antibody
      • Differentially expressed in FDCP 6 homologue mouse antibody
      • IBP antibody
      • IRF4-binding protein antibody
      • Swap70 like adapter of T cells antibody
      see all

    Anti-DEF6 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab57228 DEF6 antibody at 1ug/ml. Predicted band size of immunogen is 63 kDa

    Anti-DEF6 antibody (ab57228) 使用論文

    ab57228 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Treatment cells expressing recombitant DEF6
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Jun 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"