Anti-Deep Orange 抗体 (ab21872)

製品の概要

  • 製品名Anti-Deep Orange antibody
  • 製品の詳細
    Mouse polyclonal to Deep Orange
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    AKETASRPSDSKIRRKLWLRIAYHDIKGTNDVKKALNLLKECDLLRIEDL LPFFADFEKIDNFKEAICDALRDYNQRIQELQREMAETTEQTDRVTAELQ

    , corresponding to amino acids 771/870 of Drosophila melanogaster Deep Orange .

  • 特記事項

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 115 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Deep Orange is required for the biogenesis of eye pigment granules. It plays a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Deep Orange is expressed both maternally and zygotically in all stages of development. Highest expression is in third larval instar. It is a member of the 'granule group' of eye color genes as mutants affect deposition in pigment granules of two types of pigments, the ommochromes and drosopterins.
  • 細胞内局在Cytoplasmic; peripheral membrane protein associated with late endosomes/lysosomes.
  • 参照データベース
    • 別名
      • Deep orange protein antibody
      • dor antibody
      • dor1 antibody
      • Vacuolar protein sorting 18 antibody

    Anti-Deep Orange antibody 画像

    • All lanes : Anti-Deep Orange antibody (ab21872) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 115 kDa

    Anti-Deep Orange antibody (ab21872) 使用論文

    ab21872 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Full larvae)
    Loading amount 40 µg
    Specification Full larvae
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Verified customer

    投稿 Jan 18 2012

    Thank you for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a f...

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    Thank you for your reply. The antibody vial seems to be damaged somehow, maybe during storage or shipping. I am very sorry for this inconvenience. I do not know what exactly has happened. I suggest therefore that we do replace this vial for you. We hav...

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    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with this antibody. We had so far no complaints about ab21872. We however take everyproduct complaint very seriously, and investigate every product that we feel may not b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"