Anti-DECTIN 2 抗体 [IMG3D1] (ab107572)

製品の概要

  • 製品名Anti-DECTIN 2 antibody [IMG3D1]
    DECTIN 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [IMG3D1] to DECTIN 2
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to amino acids 75-125 of Human DECTIN 2 (Q6EIG7).

  • ポジティブ・コントロール
    • Human, mouse and rat heart lysate, human Ramos cell lysate, human PBMCs and human spleen tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名IMG3D1
  • アイソタイプIgG3
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. Use 0.5 ug/5x105 cells



ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.
  • 組織特異性Expressed in lung, spleen, lymph node, leukocytes, bone marrow, tonsils and dendritic cells. Strongly expressed in purified monocytes and weakly in B-cells. In peripheral blood cells, preferentially expressed in plasmacytoids rather than myeloids.
  • 配列類似性Contains 1 C-type lectin domain.
  • ドメインA short stretch of the intracellular domain (AA 8-14) proximal to the transmembrane domain is required for association with Fc receptor gamma chain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C type lectin domain family 6 member A antibody
    • C type lectin superfamily member 10 antibody
    • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 10 antibody
    • C-type lectin domain family 6 member A antibody
    • C-type lectin superfamily member 10 antibody
    • CLC6A_HUMAN antibody
    • CLEC4N antibody
    • CLEC4N, mouse, homolog of antibody
    • Clec6a antibody
    • CLECSF10 antibody
    • DC associated C type lectin 2 antibody
    • DC-associated C-type lectin 2 antibody
    • Dectin-2 antibody
    • Dectin2 antibody
    • Dendritic cell associated lectin 2 antibody
    • Dendritic cell-associated C-type lectin 2 antibody
    see all

Anti-DECTIN 2 antibody [IMG3D1] 画像

  • All lanes : Anti-DECTIN 2 antibody [IMG3D1] (ab107572) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate with immunizing peptide
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Human Ramos cell lysate

    Secondary
    goat anti-mouse Ig HRP

    Predicted band size : 24 kDa
  • Cell surface flow cytometric analysis of DECTIN 2 expression by 5x10^5 human PBMCs using 0.5 µg of ab107572 in red and 0.5 µg of isotype control in green.
  • Immunohistochemical analysis of DECTIN 2 in formalin-fixed, paraffin-embedded human spleen tissue using ab107572 at 5 µg/ml.

Anti-DECTIN 2 antibody [IMG3D1] (ab107572) 使用論文

ab107572 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. I am pleased to hear that enzymatic antigen retrieval seemed to help. After discussing this with a colleague I also have the following suggestions: - The blocking conditions are quite stringent therefore it is worth...

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Thank you for your patience. I think is makes sense to investigate the problems with IHC-P to see whether some protocol modifications will get the product working successfully, as we don’t know whether it is suitable for GMA embedded tissues. Would...

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Thank you for calling us today. I am sorry to hear you have been experiencing problems with this product. We have not received any other complaints about this product therefore there may be a problem with the vial received. I would be happy to offer ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"