Anti-Dectin-1 抗体 [2A11] (FITC) (ab21646)

製品の概要

  • 製品名
    Anti-Dectin-1 antibody [2A11] (FITC)
    Dectin-1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [2A11] to Dectin-1 (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant full length protein corresponding to Dectin-1.

  • 特記事項


    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2A11
  • ミエローマ
    Y3/Ag1.2.3
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells in 100µl.





ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • 組織特異性
    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • 関連疾患
    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • 配列類似性
    Contains 1 C-type lectin domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues in response to glucan binding.
  • 細胞内局在
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

Anti-Dectin-1 antibody [2A11] (FITC) 画像

  • Flow cytometry staining of mouse peripheral blood granulocytes with ab21646.

プロトコール

Anti-Dectin-1 antibody [2A11] (FITC) (ab21646) 使用論文

ab21646 has not yet been referenced specifically in any publications.

Product Wall


Information we have from the clone originators indicates the antibody recognises both isoforms.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (RAW and J774 murine macrophages)
Specification
RAW and J774 murine macrophages
Preparation
Cell harvesting/tissue preparation method: routine cell culture --> scraped, washed
Sample buffer: PBS + FBS (3%)
Fixation
None
Permeabilization
No
Gating Strategy
Size gating of murine RAW cells
Username

Abcam user community

Verified customer

投稿 May 23 2008

Thank you for your enquiry. The antibody is specific for Dectin-1. This is the only antibody we carry for this particular target. The references listed on the datasheet are general references pertaining to the target of the antibody. They are n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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