製品の概要

  • 製品名Anti-Dectin-1 antibody [2A11]
    Dectin-1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [2A11] to Dectin-1
  • アプリケーション適用あり: IP, IHC-Fr, Flow Cytmore details
    適用なし: IHC-P or IHC-R
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Dectin-1.

  • 特記事項


    ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 一次抗体 備考ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
  • ポリ/モノモノクローナル
  • クローン名2A11
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

The epitope recognised by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody.

Flow Cyt 1/10 - 1/50.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P or IHC-R.
  • ターゲット情報

    • 機能Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
    • 組織特異性Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
    • 関連疾患Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
    • 配列類似性Contains 1 C-type lectin domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues in response to glucan binding.
    • 細胞内局在Cytoplasm and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta GR antibody
      • Beta-glucan receptor antibody
      • BGR antibody
      • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
      • C type lectin domain family 7 member a antibody
      • C-type lectin domain family 7 member A antibody
      • C-type lectin superfamily member 12 antibody
      • CANDF4 antibody
      • CLC7A_HUMAN antibody
      • CLEC7A antibody
      • CLEC7A protein antibody
      • Clecsf12 antibody
      • DC-associated C-type lectin 1 antibody
      • Dectin 1 antibody
      • DECTIN 1 receptor antibody
      • Dectin-1 antibody
      • Dectin1 antibody
      • Dendritic cell associated C type lectin 1 antibody
      • Dendritic cell-associated C-type lectin 1 antibody
      • Lectin like receptor 1 antibody
      • Putative transmembrane protein dectin 1 antibody
      see all

    Anti-Dectin-1 antibody [2A11] 画像

    • Staining with Rat anti mouse beta Glucan Receptor. (ab53427).

    Anti-Dectin-1 antibody [2A11] (ab53427) 使用論文

    This product has been referenced in:
    • Seifert L  et al. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Rep 13:1909-21 (2015). Flow Cyt ; Mouse . Read more (PubMed: 26655905) »

    See 1 Publication for this product

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    Thank you for your inquiry.

    I am sorry the epitope of this antibody hasn't been determined yet. The immunogen of this antibody corresponds to recombinant beta Glucan Receptor protein.

    I hope this information is helpful to you. Ple...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"