Anti-DECR1 抗体 [8B1AD10] (ab110287)
Key features and details
- Mouse monoclonal [8B1AD10] to DECR1
- Suitable for: IP, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-DECR1 antibody [8B1AD10]
DECR1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8B1AD10] to DECR1 -
由来種
Mouse -
アプリケーション
適用あり: IP, ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HeLa cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
特記事項(精製)
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
8B1AD10 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110287の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria.
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IP
Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. -
組織特異性
Heart = liver = pancreas > kidney >> skeletal muscle = lung. -
配列類似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 1666 Human
- Omim: 222745 Human
- SwissProt: Q16698 Human
- Unigene: 492212 Human
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別名
- 1200012F07Rik antibody
- 2 antibody
- 2,4 dienoyl CoA reductase [NADPH] antibody
see all
画像
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DECR1 immunocaptured from human liver mitochondria using ab110287.
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HeLa cells were stained with 1 µg/mL ab110287 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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ab110287 staining DECR1 in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry. DECR1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
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MRC5 fibroblasts labeled with 1 µg/ml ab110287 and detected with goat anti-mouse secondary conjugated to FITC.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110287 は論文での使用が確認できていません。