製品の概要

  • 製品名Anti-DDX6 antibody
    DDX6 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to DDX6
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Mouse, Guinea pig, Dog
  • 免疫原

    Synthetic peptide corresponding to Human DDX6 aa 471-483 (C terminal).
    Sequence:

    C-AEYHSEPVEDEKP


    Database link: P26196

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
    • 細胞内局在Cytoplasm; P-body. Note: Processing bodies (PB).
    • 参照データベース
    • 別名
      • ATP dependent RNA helicase DDX6 antibody
      • ATP dependent RNA helicase p54 antibody
      • DDX6 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
      • DEAD box 6 antibody
      • DEAD box protein 6 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
      • FLJ36338 antibody
      • HLR2 antibody
      • Oncogene RCK antibody
      • P54 antibody
      • Probable ATP-dependent RNA helicase DDX6 antibody
      • RCK antibody
      see all

    Anti-DDX6 antibody 画像

    • All lanes : Anti-DDX6 antibody (ab45869) at 0.3 µg/ml

      Lane 1 : Human Burkitt's lymphoma cell line
      Lane 2 : Human T cell leukemia cells from peripheral blood
      Lane 3 : Human liver hepatocellular carcinoma

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 54 kDa
    • Anti-DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Primary incubation was 1 hour. Detected by chemiluminescence.

    Anti-DDX6 antibody (ab45869) 使用論文

    This product has been referenced in:
    • Shen X  et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB ; Pig . Read more (PubMed: 19299420) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab45869.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"