製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DDX56
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 251-403 of Human DDX21 (AAH01235).

  • ポジティブ・コントロール
    • A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
  • 組織特異性
    Detected in heart, brain, liver, pancreas, placenta and lung.
  • 配列類似性
    Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 61 kd nucleolar helicase antibody
    • ATP-dependent 61 kDa nucleolar RNA helicase antibody
    • DDX21 antibody
    • DDX26 antibody
    • Ddx56 antibody
    • DDX56_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 56 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
    • DEAD box protein 21 antibody
    • DEAD box protein 56 antibody
    • DEAD box RNA helicase antibody
    • DEAD/H box 56 antibody
    • NOH61 antibody
    • Nucleolar helicase of 61 kDa antibody
    • Probable ATP-dependent RNA helicase DDX56 antibody
    • Putative nucleolar RNA helicase antibody
    see all

画像

  • Anti-DDX56 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 62 kDa
  • ab97648, at a 1/200 dilution, staining DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
  • ab97648, at a 1/500 dilution, staining DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.

参考文献

ab97648 has not yet been referenced specifically in any publications.

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