製品の概要

  • 製品名Anti-DDX56 antibody
    DDX56 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDX56
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) corresponding to a region between residue 300 and 350 of DDX56 according to NP_061955.1 (GeneID 54606).

  • ポジティブ・コントロール
    • HeLa whole cell lysate, 293T whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab115178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
  • 組織特異性Detected in heart, brain, liver, pancreas, placenta and lung.
  • 配列類似性Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 61 kd nucleolar helicase antibody
    • ATP-dependent 61 kDa nucleolar RNA helicase antibody
    • DDX21 antibody
    • DDX26 antibody
    • Ddx56 antibody
    • DDX56_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 56 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
    • DEAD box protein 21 antibody
    • DEAD box protein 56 antibody
    • DEAD box RNA helicase antibody
    • DEAD/H box 56 antibody
    • NOH61 antibody
    • Nucleolar helicase of 61 kDa antibody
    • Probable ATP-dependent RNA helicase DDX56 antibody
    • Putative nucleolar RNA helicase antibody
    see all

Anti-DDX56 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115178 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX56 antibody (ab115178) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 30 seconds
  • Detection of DDX56 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.

Anti-DDX56 antibody (ab115178) 使用論文

ab115178 has not yet been referenced specifically in any publications.

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