The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Use at 2-5 µg/mg of lysate.
DDX51 belongs to the DEAD box helicase family. It is thought to be an ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
DEAD (Asp Glu Ala Asp) box polypeptide 51 antibody
DEAD box protein 51 antibody
Dead box protein 73D like antibody
Western blot - DDX51 antibody (ab72292)
All lanes : Anti-DDX51 antibody (ab72292) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa Observed band size: 72 kDa Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation - DDX51 antibody (ab72292)
Detection of DDX51 by Western Blot of Immunprecipitate.
Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 minutes.