製品の概要

  • 製品名Anti-DDX51 antibody
    DDX51 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DDX51
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide cprresponding to a region between residues 616 and 666 of human DDX51 (NP_778236.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性DDX51 belongs to the DEAD box helicase family. It is thought to be an ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Helicases are involved in unwinding nucleic acids. The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
  • 細胞内局在Nuclear, nucleolus.
  • 参照データベース
  • 別名
    • DDX 51 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 51 antibody
    • DEAD box protein 51 antibody
    • Dead box protein 73D like antibody
    • MGC42193 antibody
    see all

Anti-DDX51 antibody 画像

  • All lanes : Anti-DDX51 antibody (ab72292) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of DDX51 by Western Blot of Immunprecipitate.
    Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-DDX51 antibody (ab72292) 使用論文

ab72292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"