Anti-DDX5 抗体 [2257C3a] (ab53216)
Key features and details
- Mouse monoclonal [2257C3a] to DDX5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-DDX5 antibody [2257C3a]
DDX5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2257C3a] to DDX5 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment of human DDX5.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035), NIH3T3 whole cell lysate and F2408 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2257C3a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53216の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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特記事項 |
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WB
1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
ターゲット情報
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機能
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
配列類似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Arg-502 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Entrez Gene: 287765 Rat
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
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別名
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab53216 observed at 69 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab53216 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab53216 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 69 kDa
データシートおよび資料
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Datasheet download
参考文献 (3)
ab53216 は 3 報の論文で使用されています。
- Xu J et al. The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NF?B transcripts to dampen antiviral innate immunity. PLoS Pathog 17:e1009530 (2021). PubMed: 33909701
- Pettersson OJ et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). PubMed: 24792155
- Yao H et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). PubMed: 20966046