製品の概要

  • 製品名Anti-DDX43 antibody
    DDX43 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DDX43
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: SHHGGAPKAS TWVVASRRSS TVSRAPERRP AEELNRTGPE GYSVGRGGRW RGTSRPPEAV AAGHEELPLC FALKSHFVGA VIGRGGSKI, corresponding to amino acids 2-90 of Human DDX43 (NP_061135), with a proprietary tag.

  • ポジティブ・コントロール
    • DDX43-transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 組織特異性Expressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.
  • 配列類似性Belongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 KH domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 13 antibody
    • CT13 antibody
    • DDX43 antibody
    • DDX43_HUMAN antibody
    • DEAD box protein 43 antibody
    • DEAD box protein HAGE antibody
    • HAGE antibody
    • Helical antigen antibody
    • Probable ATP dependent RNA helicase DDX43 antibody
    • Probable ATP-dependent RNA helicase DDX43 antibody
    see all

Anti-DDX43 antibody 画像

  • All lanes : Anti-DDX43 antibody (ab77416) at 5 µg/ml

    Lane 1 : DDX43-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • Anti-DDX43 antibody (ab77416) at 5 µg/ml + immunogen (tagged 88 aa recombinant fragment) at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-DDX43 antibody (ab77416) 使用論文

ab77416 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77416.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"