製品の概要

  • 製品名
    Anti-DDX4 / MVH antibody [mAbcam27591]
    DDX4 / MVH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam27591] to DDX4 / MVH
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rabbit, Human, Pig, Common marmoset
  • 免疫原

    Synthetic peptide corresponding to Human DDX4/ MVH aa 700 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: Mouse testis tissue lysate and mouse EG whole cell lysate. IHC-P: Human normal testis tissue. ICC/IF: mES cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 0.05 - 0.1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 76 kDa).

ターゲット情報

  • 機能
    Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
  • 組織特異性
    Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
  • 配列類似性
    Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DDX 4 antibody
    • Ddx4 antibody
    • DDX4_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
    • DEAD box protein 4 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
    • MVH antibody
    • Probable ATP dependent RNA helicase DDX4 antibody
    • Probable ATP-dependent RNA helicase DDX4 antibody
    • VASA antibody
    • Vasa homolog antibody
    see all

Anti-DDX4 / MVH antibody [mAbcam27591] 画像

  • ab27591 staining DDX4 / MVH antibody in pig tissue sections (testis, postnatal day 42) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% 0.1% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. Ab150110l at a dilution of 1/500 was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591) at 5 µg/ml (Blocked with 3% Milk)

    Lane 1 : Mouse Testis Tissue Lysate
    Lane 2 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minuteAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • IHC-P image of DDX4 / MVH (ab27591) on adult marmoset testis. The sections were fixed with Formaldehyde and blocked in 5% milk for 30 minutes, before incubation with the DDX4 antibody (1/50 dilution) for 18 hours at 4°C. Cytoplasmic staining is seen with ab27591 (green), DAPI was used to stain nuclei (blue).

    See Abreview

  • ICC/IF image of ab27591 stained mES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27591, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC-Fr image of DDX4 (ab27591) on mouse embryonic gonad cells. The sections were fixed with 4% PFA, permeabilized and blocked in BSA, before incubation with the DDX4 antibody (1/100 dilution) for 12 hours at 4 degrees. Cytoplasmic staining of the mouse primordial germ cells by ab27591 is shown in red and Oct4-GFP staining is shown in green.

    See Abreview

  • IHC image of DDX4/MVH staining in human normal Testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27591, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591) 使用論文

This product has been referenced in:
  • Lopes F  et al. Irinotecan metabolite SN38 results in germ cell loss in the testis but not in the ovary of prepubertal mice. Mol Hum Reprod 22:745-755 (2016). IHC-P ; Mouse . Read more (PubMed: 27470502) »
  • Saatcioglu HD  et al. Control of Oocyte Reawakening by Kit. PLoS Genet 12:e1006215 (2016). Read more (PubMed: 27500836) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Testis, postnatal day 42)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 42
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Apr 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Apr 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate 0,05% Tween 20
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 28 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Chimpanzee Tissue sections (adult testis)
Specification
adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

煜欽 林

Verified customer

投稿 Dec 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

投稿 Jan 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 Male Gonad)
Specification
E12.5 Male Gonad
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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