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Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human DDX4/MVH.
(Peptide available as ab13841.)
Our Abpromise guarantee covers the use of ab13840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23598447
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/200. PubMed: 19440488|
|IHC-Fr||Use a concentration of 1 µg/ml.|
Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1
The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
ab13840 at 1/1000 staining germ cells of the embryonic mouse testis by ICC/IF. Cells were PFA fixed and permeabilized in 1% Triton X in PBS prior to blocking in 5% serum for 2 hours at 20°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.
Ab13840 at 1/400 staining mouse fetal testis tissue sections by IHC-P. The tissue was blocked with both BSA and serum and fixed prior to incubation with the antibody for 3 hours at room temperature. A biotinylated secondary antibody was used (Vectastain Elite).
Please note Bouin's fluid was used as the fixative in this experiment.
ab13840 staining Postnatal Porcine Testis tissue sections by IHC-P. Bouin's Fixative was used to fix the sections prior to a heat mediated antigen retrieval step in sodium citrate buffer (pH 6). The sample was blocked in 10% serum for 1 hour at 22°C prior to incubation with the primary antibody, diluted 1/500, for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary
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